Genotypic characterization of Streptococcus pneumoniae serotype 19F in Malaysia

Jayakayatri Jeevajothi Nathan; Mohd Nasir Mohd Desa; Kwai Lin Thong; Stuart C Clarke; Siti Norbaya Masri; Rohani Md Yasin; Niazlin Mohd Taib

doi:10.1016/j.meegid.2013.11.026

Genotypic characterization of Streptococcus pneumoniae serotype 19F in Malaysia

Infect Genet Evol. 2014 Jan:21:391-4. doi: 10.1016/j.meegid.2013.11.026. Epub 2013 Dec 14.

Authors

Jayakayatri Jeevajothi Nathan¹, Mohd Nasir Mohd Desa², Kwai Lin Thong³, Stuart C Clarke⁴, Siti Norbaya Masri⁵, Rohani Md Yasin⁶, Niazlin Mohd Taib⁷

Affiliations

¹ Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia. Electronic address: jjayakayatri@yahoo.com.
² Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia. Electronic address: mnasir@upm.edu.my.
³ Division of Microbiology, Institute of Biological Science, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia; Biomedical Science and Molecular Typing Laboratory, Institute of Graduate Studies, University of Malaya, 50603 Kuala Lumpur, Malaysia.
⁴ Infectious Disease Epidemiology Group, Sir Henry Wellcome Laboratories, Academic Unit of Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, UK; NIHR Southampton Respiratory Biomedical Research Unit, Southampton, UK; Institute of Life Sciences, University of Southampton, UK; Public Health England, Southampton, UK.
⁵ Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia. Electronic address: sitinorbaya@upm.edu.my.
⁶ Specialized Diagnostic Centre, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia.
⁷ Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia. Electronic address: niazlin@upm.edu.my.

PMID: 24342879
DOI: 10.1016/j.meegid.2013.11.026

Abstract

Streptococcus pneumoniae is an epidemiologically important bacterial pathogen. Recently, we reported the antibiotic susceptibility patterns of a limited collection of pneumococcal isolates in Malaysia with a high prevalence of erythromycin resistant strains. In the present study, 55 of the pneumococcal isolates of serotype 19F were further analysed by pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The generated genotypic patterns were then correlated with the antibiograms previously reported. Forty-seven different PFGE profiles (PTs) were obtained, showing that the isolates were genetically diverse. MLST identified 16 sequence types (STs) with ST-236 being predominant (58.2%), followed by ST-81 (10.3%). Among the ST-236 isolates, 22 were erythromycin resistant S. pneumoniae (ERSP) and 15 were trimethoprim/sulfamethoxazole (TMP/SMX) resistant, while among ST-81, four isolates were ERSP and two were TMP/SMX resistant. The high prevalence of erythromycin resistant serotype 19F isolates of ST-236 in this study has also been reported in other North and South East Asian countries.

Keywords: Antibiogram; Multilocus sequence type; Pulsed field gel electrophoresis; Serotype 19F; Streptococcus pneumoniae.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology
DNA, Bacterial / analysis
Drug Resistance, Multiple, Bacterial / genetics*
Electrophoresis, Gel, Pulsed-Field
Genotype
Humans
Malaysia / epidemiology
Multilocus Sequence Typing
Phylogeny
Pneumococcal Infections / epidemiology*
Pneumococcal Infections / microbiology*
Streptococcus pneumoniae / drug effects
Streptococcus pneumoniae / genetics*
Streptococcus pneumoniae / isolation & purification

Substances

Anti-Bacterial Agents
DNA, Bacterial