Efficient expression and purification of Fc-fragment-binding domain and its application to immunoglobulin G purification

Biomed Environ Sci. 2013 Nov;26(11):916-9. doi: 10.3967/bes2013.021.

Authors

Xing Zhen Lao¹, Ya Li Zhou, Heng Zheng

Affiliation

¹ School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, Jiangsu, China.

PMID: 24331537
DOI: 10.3967/bes2013.021

No abstract available

Publication types

Letter
Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Binding Sites, Antibody
Biochemistry / methods*
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Escherichia coli / genetics
Genetic Vectors
Immunoglobulin Fc Fragments / genetics
Immunoglobulin Fc Fragments / metabolism*
Immunoglobulin G / genetics
Immunoglobulin G / metabolism*
Ligands
Molecular Sequence Data
Plasmids
Protein Binding
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / isolation & purification*
Staphylococcal Protein A / genetics
Staphylococcal Protein A / metabolism

Substances

Immunoglobulin Fc Fragments
Immunoglobulin G
Ligands
Recombinant Fusion Proteins
Staphylococcal Protein A