The effect of timing of gamma-irradiation on hemolysis and potassium release in leukoreduced red cell concentrates stored in SAGM

K Serrano; D Chen; A L Hansen; E Levin; T R Turner; J D R Kurach; J P Acker; D V Devine

doi:10.1111/vox.12112

The effect of timing of gamma-irradiation on hemolysis and potassium release in leukoreduced red cell concentrates stored in SAGM

Vox Sang. 2014 May;106(4):379-81. doi: 10.1111/vox.12112. Epub 2013 Dec 12.

Authors

K Serrano¹, D Chen, A L Hansen, E Levin, T R Turner, J D R Kurach, J P Acker, D V Devine

Affiliation

¹ Canadian Blood Services, Centre for Innovation, Vancouver, BC, Canada; Centre for Blood Research, University of British Columbia, Vancouver, BC, Canada; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada.

PMID: 24330144
DOI: 10.1111/vox.12112

Abstract

While irradiation of red cell concentrates (RCC) prevents graft-versus-host disease in susceptible transfusion recipients, it also damages red blood cells (RBC). To understand the ability of irradiation regulations to prevent transfusion of inferior units, we irradiated 980 RCC in saline-adenine-glucose-mannitol (SAGM) using various combinations of pre-irradiation age and post-irradiation storage times, and measured hemolysis and extracellular potassium levels. We observed unacceptably high hemolysis (>0·8%) in some RCC and elevated extracellular potassium levels in all gamma-irradiated RCC. This suggests that more restrictive storage times should be considered for RCC in SAGM.

Keywords: blood components; component production; quality control.

MeSH terms

Adenine / chemistry
Blood Safety*
Blood Transfusion
Erythrocytes / radiation effects*
Gamma Rays*
Glucose / chemistry
Graft vs Host Disease / prevention & control
Hemolysis / radiation effects*
Humans
Mannitol / chemistry
Potassium / blood*
Sodium Chloride / chemistry
Solutions
Time Factors

Substances

Solutions
Mannitol
Sodium Chloride
Glucose
Adenine
Potassium