Monoclonal antibodies against NS4B protein of japanese encephalitis virus

Monoclon Antib Immunodiagn Immunother. 2013 Dec;32(6):382-5. doi: 10.1089/mab.2013.0041.

Abstract

Japanese encephalitis (JE) is one of the most prevalent global viral encephalitis viruses. The functions of JEV (virus) NS4B protein are still under investigation. In our study, NS4B was expressed in Escherichia coli and purified by dialysis. Two clones of monoclonal antibodies (MAbs 1B1 and 1C3) against NS4B protein were generated and their characterizations were investigated. IFA, Western blot, and ELISA results showed that the MAbs were specific against JEV NS4B protein. The epitope of the MAbs was further identified using pairs of synthesized overlapping peptides. These MAbs may provide valuable tools for further exploration of the functions of NS4B and the pathogenesis of Japanese encephalitis virus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal, Murine-Derived / biosynthesis
  • Antibodies, Monoclonal, Murine-Derived / chemistry*
  • Antibodies, Monoclonal, Murine-Derived / immunology
  • Antibody Specificity
  • Blotting, Western
  • Cell Line
  • Cricetinae
  • Encephalitis Virus, Japanese / immunology
  • Encephalitis Virus, Japanese / metabolism*
  • Enzyme-Linked Immunosorbent Assay
  • Epitope Mapping
  • Female
  • Fluorescent Antibody Technique, Indirect
  • Hybridomas
  • Immunoglobulin G / biosynthesis
  • Immunoglobulin G / chemistry*
  • Immunoglobulin G / immunology
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Viral Nonstructural Proteins / chemistry
  • Viral Nonstructural Proteins / immunology*
  • Viral Nonstructural Proteins / metabolism

Substances

  • Antibodies, Monoclonal, Murine-Derived
  • Immunoglobulin G
  • NS4B protein, flavivirus
  • Viral Nonstructural Proteins