Abstract
A peptide that binds and emits fluorescence in response to conformational change in a target protein was developed by in vitro selection using tRNA carrying a fluorogenic amino acid. This technology could prove to be useful for the development of separation-free immunoassays and bio-imaging analyses.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acids / chemistry
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Amino Acids / metabolism*
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Azoles / chemistry
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Calmodulin / metabolism*
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Fluorescent Dyes / chemistry
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Fluorescent Dyes / metabolism*
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Nitrobenzenes / chemistry
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Peptides / chemistry
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Peptides / metabolism*
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Protein Binding
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RNA, Transfer
Substances
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7-nitrobenz-2-oxa-1,3-diazol-4-yl
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Amino Acids
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Azoles
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Calmodulin
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Fluorescent Dyes
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Nitrobenzenes
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Peptides
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RNA, Transfer