A fluorogenic peptide probe developed by in vitro selection using tRNA carrying a fluorogenic amino acid

Chem Commun (Camb). 2014 Mar 18;50(22):2962-4. doi: 10.1039/c3cc47624c. Epub 2013 Dec 9.

Abstract

A peptide that binds and emits fluorescence in response to conformational change in a target protein was developed by in vitro selection using tRNA carrying a fluorogenic amino acid. This technology could prove to be useful for the development of separation-free immunoassays and bio-imaging analyses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids / chemistry
  • Amino Acids / metabolism*
  • Azoles / chemistry
  • Calmodulin / metabolism*
  • Fluorescent Dyes / chemistry
  • Fluorescent Dyes / metabolism*
  • Nitrobenzenes / chemistry
  • Peptides / chemistry
  • Peptides / metabolism*
  • Protein Binding
  • RNA, Transfer

Substances

  • 7-nitrobenz-2-oxa-1,3-diazol-4-yl
  • Amino Acids
  • Azoles
  • Calmodulin
  • Fluorescent Dyes
  • Nitrobenzenes
  • Peptides
  • RNA, Transfer