Screening of various botanical extracts for antioxidant activity using DPPH free radical method

Afr J Tradit Complement Altern Med. 2013 Oct 3;10(6):452-5. doi: 10.4314/ajtcam.v10i6.9. eCollection 2013.

Abstract

Aiming at the exploration of herbal use by society, crude extracts of the seeds of some commonly used medicinal plants (Vitis vinifera, Tamarindus indica and Glycin max) were screened for their free radical scavenging properties using ascorbic acid as standard antioxidant. Free radical scavenging activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical. The overall antioxidant activity of grape seeds (Vitis vinifera) was the strongest, followed in descending order by soybean (Glycin max) and tamarind (Tamarindus indica). The seeds extract of Vitis vinifera, Glycin max and Tamarindus indica showed 85.61%, 83.45% and 79.26%, DPPH scavenging activity respectively.

Keywords: Antioxidant activity; DPPH; Glycin max; Tamarindus indica; Vitis vinifera; free-radical.

MeSH terms

  • Antioxidants / analysis
  • Antioxidants / chemistry
  • Biphenyl Compounds
  • Free Radical Scavengers / analysis*
  • Free Radical Scavengers / chemistry
  • Free Radicals
  • Glycine max / chemistry*
  • Indicators and Reagents
  • Picrates
  • Plant Extracts / analysis*
  • Plant Extracts / chemistry
  • Seeds*
  • Tamarindus / chemistry*
  • Vitis / chemistry*

Substances

  • Antioxidants
  • Biphenyl Compounds
  • Free Radical Scavengers
  • Free Radicals
  • Indicators and Reagents
  • Picrates
  • Plant Extracts
  • 1,1-diphenyl-2-picrylhydrazyl