Micropatterned polymeric nanosheets for local delivery of an engineered epithelial monolayer

Adv Mater. 2014 Mar 19;26(11):1699-705. doi: 10.1002/adma.201304183. Epub 2013 Dec 4.

Abstract

Like a carpet for cells, micropatterned polymeric nanosheets are developed toward local cell delivery. The nanosheets direct morphogenesis of retinal pigment epithelial (RPE) cells and allow for the injection of an engineered RPE monolayer through syringe needles without the loss of cell viability. Such an ultrathin carrier has the promise of a minimally invasive delivery of cells into narrow tissue spaces.

Keywords: cell delivery; magnetic nanoparticles (MNP); retinal pigment epithelium (RPE); tight junctions; ultrathin films (nanosheets).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Absorbable Implants
  • Animals
  • Cell Culture Techniques
  • Cell Line
  • Cell Survival
  • In Vitro Techniques
  • Macula Lutea / physiology
  • Macular Degeneration
  • Nanostructures / chemistry
  • Nanostructures / therapeutic use*
  • Ophthalmologic Surgical Procedures
  • Pliability
  • Polymers / chemistry
  • Rats
  • Rats, Long-Evans
  • Retinal Pigment Epithelium / cytology
  • Retinal Pigment Epithelium / physiology
  • Retinal Pigment Epithelium / transplantation*
  • Stress, Mechanical
  • Swine
  • Syringes
  • Tight Junctions / physiology
  • Tissue Engineering / methods*
  • Tissue Scaffolds*
  • Zonula Occludens-1 Protein / metabolism

Substances

  • Polymers
  • Tjp1 protein, rat
  • Zonula Occludens-1 Protein