Bromo- and thiomaleimides as a new class of thiol-mediated fluorescence 'turn-on' reagents

Org Biomol Chem. 2014 Jan 28;12(4):557-60. doi: 10.1039/c3ob42141d.

Abstract

Bromo- and thiomaleimides are shown to serve as highly effective quenchers of a covalently attached fluorophore. Reactions with thiols that lead to removal of the maleimide conjugation, or detachment of the fluorophore from the maleimide, result in 'turn-on' of the fluorescence. These reagents thus offer opportunities in thiol sensing and intracellular reporting.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry*
  • HEK293 Cells
  • Humans
  • Maleimides / chemical synthesis
  • Maleimides / chemistry*
  • Molecular Structure
  • Sulfhydryl Compounds / chemistry*

Substances

  • Fluorescent Dyes
  • Maleimides
  • Sulfhydryl Compounds