Analytic validation and comparison of three commercial immunoassays for measurement of plasma atrial/A-type natriuretic peptide concentration in horses

Abstract

Measurement of atrial/A-type natriuretic peptide (ANP) concentrations may be of use for assessment of cardiac disease, and reliable data on the analytic performance of available assays are needed. To assess the suitability for clinical use of commercially available ANP assays, intra-assay and inter-assay coefficient of variation and dilution parallelism were calculated for three immunoassays (RIAPen, RIAPhoen, and an ELISAPen) using blood samples from healthy and diseased horses to cover a wide range of ANP concentrations. Further, agreement between assays was assessed using linear regression and Bland-Altman analyses. For all assays, precision was moderate but acceptable and dilution parallelism was good. All assays showed analytic performance similar to other immunoassays used in veterinary medicine. However, the results from the three assays were poorly comparable. Our study highlights the need for an optimised species-specific assay for equine samples.

Keywords: AB; AG; ANP; Accuracy; BNP; Biomarker; CV; Cardiac; ELISA; Equine; N-terminal pro brain/B-type natriuretic peptide; NT-proBNP; Precision; RIA; Radioimmunoassay; antibody; antigen; atrial/A-type natriuretic peptide; brain/B-type natriuretic peptide; coefficient of variation; enzyme-linked immunosorbent assay.