Live cell visualization of Golgi membrane dynamics by super-resolution confocal live imaging microscopy

Methods Cell Biol. 2013:118:235-42. doi: 10.1016/B978-0-12-417164-0.00014-8.

Abstract

Super-resolution confocal live imaging microscopy we developed provides cutting-edge high-speed live cell imaging at high space resolution. With this technology we are now able to observe details of membrane traffic events, including behaviors of small vesicles, cisternal maturation of the Golgi apparatus, and membrane segregation within a compartment.

Keywords: Cisternal maturation; Live imaging; Multicolor visualization; Spinning disk confocal; Super-resolution.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Golgi Apparatus / metabolism*
  • Microscopy, Confocal
  • Microscopy, Fluorescence
  • Protein Transport
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae / ultrastructure*
  • Saccharomyces cerevisiae Proteins / metabolism
  • Single-Cell Analysis

Substances

  • Saccharomyces cerevisiae Proteins