IGZO thin film transistor biosensors functionalized with ZnO nanorods and antibodies

Yi-Chun Shen; Chun-Hsu Yang; Shu-Wen Chen; Shou-Hao Wu; Tsung-Lin Yang; Jian-Jang Huang

doi:10.1016/j.bios.2013.10.043

IGZO thin film transistor biosensors functionalized with ZnO nanorods and antibodies

Biosens Bioelectron. 2014 Apr 15:54:306-10. doi: 10.1016/j.bios.2013.10.043. Epub 2013 Nov 12.

Authors

Yi-Chun Shen¹, Chun-Hsu Yang¹, Shu-Wen Chen¹, Shou-Hao Wu¹, Tsung-Lin Yang², Jian-Jang Huang³

Affiliations

¹ Graduate Institute of Photonics and Optoelectronics, National Taiwan University, 1, Roosevelt Road, Section 4, Taipei 106, Taiwan.
² Department of Otolaryngology, National Taiwan University Hospital and College of Medicine, National Taiwan University, No. 1, Jen-Ai Road, Section 1, Taipei 100, Taiwan; Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, No. 1, Jen-Ai Road, Section 1, Taipei 100, Taiwan. Electronic address: tlyang@ntu.edu.tw.
³ Graduate Institute of Photonics and Optoelectronics, National Taiwan University, 1, Roosevelt Road, Section 4, Taipei 106, Taiwan; Department of Electrical Engineering, National Taiwan University, 1, Roosevelt Road, Section 4, Taipei 106, Taiwan. Electronic address: jjhuang@cc.ee.ntu.edu.tw.

PMID: 24291267
DOI: 10.1016/j.bios.2013.10.043

Abstract

We demonstrate a biosensor structure consisting of an IGZO (Indium-Gallium-Zinc-Oxide) TFT (thin film transistor) and an extended sensing pad. The TFT acts as the sensing and readout device, while the sensing pad ensures the isolation of biological solution from the transistor channel layer, and meanwhile increases the sensing area. The biosensor is functionalized by first applying ZnO nanorods to increase the surface area for attracting electrical charges of EGFR (epidermal growth factor receptor) antibodies. The device is able to selectively detect 36.2 fM of EGFR in the total protein solution of 0.1 ng/ml extracted from squamous cell carcinoma (SCC). Furthermore, the conjugation duration of the functionalized device with EGFR can be limited to 3 min, implying that the biosensor has the advantage for real-time detection.

Keywords: Biosensors; Nanorods; Thin film transistors.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Immobilized / chemistry*
Biosensing Techniques / instrumentation*
Cell Line
Cell Line, Tumor
Equipment Design
Gallium / chemistry
Humans
Indium / chemistry
Limit of Detection
Nanotubes / chemistry*
Transistors, Electronic*
Zinc Oxide / chemistry*

Substances

Antibodies, Immobilized
Indium
gallium oxide
indium oxide
Gallium
Zinc Oxide