Influence of lysophosphatidic acid on estradiol production and follicle stimulating hormone action in bovine granulosa cells

Reprod Biol. 2013 Dec;13(4):344-7. doi: 10.1016/j.repbio.2013.09.004. Epub 2013 Oct 8.

Abstract

The objective of the study was to examine the effect of lysophosphatidic acid (LPA) on 17β-estradiol (E2) synthesis and follicle stimulating hormone (FSH) action in bovine granulosa cells. We found that granulosa cells in the bovine antral follicle, in addition to the uterus and the CL, are also the site of LPA synthesis and the target for LPA action in the bovine reproductive tract. Our findings suggest that LPA stimulates E2 synthesis, probably via increased expression of FSHR and 17β-HSD genes.

Keywords: Autotaxin; Cow; Estradiol; Follicle stimulating hormone; Granulosa cells; LPAR; Lysophosphatidic acid; Phospholipase A2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Corpus Luteum / drug effects
  • Corpus Luteum / metabolism
  • DNA Primers / genetics
  • Estradiol / biosynthesis*
  • Female
  • Follicle Stimulating Hormone / metabolism*
  • Gene Expression Regulation, Developmental / drug effects*
  • Granulosa Cells / drug effects
  • Granulosa Cells / metabolism*
  • Lysophospholipids / pharmacology*
  • Real-Time Polymerase Chain Reaction / veterinary
  • Uterus / drug effects
  • Uterus / metabolism

Substances

  • DNA Primers
  • Lysophospholipids
  • Estradiol
  • Follicle Stimulating Hormone
  • lysophosphatidic acid