A novel Cry9Aa with increased toxicity for Spodoptera exigua (Hübner)

Samir Naimov; Radostina Nedyalkova; Nikola Staykov; Mieke Weemen-Hendriks; Ivan Minkov; Ruud A de Maagd

doi:10.1016/j.jip.2013.11.003

A novel Cry9Aa with increased toxicity for Spodoptera exigua (Hübner)

J Invertebr Pathol. 2014 Jan:115:99-101. doi: 10.1016/j.jip.2013.11.003. Epub 2013 Nov 25.

Authors

Samir Naimov¹, Radostina Nedyalkova², Nikola Staykov², Mieke Weemen-Hendriks³, Ivan Minkov², Ruud A de Maagd³

Affiliations

¹ University of Plovdiv "Paisii Hilendarski", 4000 Plovdiv, Bulgaria. Electronic address: naimov0@uni-plovdiv.bg.
² University of Plovdiv "Paisii Hilendarski", 4000 Plovdiv, Bulgaria.
³ Business Unit Bioscience, Plant Research International, 6700 AP Wageningen, The Netherlands.

PMID: 24286660
DOI: 10.1016/j.jip.2013.11.003

Abstract

Cry9Aa, produced by Bacillus thuringiensis is reported to be not active against Spodoptera exigua (beet armyworm). In this study we have cloned a new cry9Aa5 gene encoding a protoxin with increased activity against S. exigua as compared to Cry9Aa1. When aligned to Cry9Aa1, four amino acid substitutions in domain I and one substitution in the C-terminal protein extension of Cry9Aa5 were identified. Toxicity of Cry9Aa5, produced in recombinant Escherichia coli was assessed and compared to the activity of Cry9Aa1, produced under the same conditions.

Keywords: Bacillus thuringiensis; Cry9Aa5; Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Bacillus thuringiensis / genetics
Bacillus thuringiensis Toxins
Bacterial Proteins / genetics*
Endotoxins / genetics*
Hemolysin Proteins / genetics*
Pest Control, Biological / methods*
Reverse Transcriptase Polymerase Chain Reaction
Spodoptera / microbiology*

Substances

Bacillus thuringiensis Toxins
Bacterial Proteins
Endotoxins
Hemolysin Proteins
insecticidal crystal protein, Bacillus Thuringiensis