A brief review of methods for terminal functionalization of DNA

Yuanchen Dong; Dongsheng Liu; Zhongqiang Yang

doi:10.1016/j.ymeth.2013.11.004

A brief review of methods for terminal functionalization of DNA

Methods. 2014 May 15;67(2):116-22. doi: 10.1016/j.ymeth.2013.11.004. Epub 2013 Nov 21.

Authors

Yuanchen Dong¹, Dongsheng Liu¹, Zhongqiang Yang²

Affiliations

¹ Key Laboratory of Organic Optoelectronics & Molecular Engineering of the Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, China.
² Key Laboratory of Organic Optoelectronics & Molecular Engineering of the Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, China. Electronic address: zyang@tsinghua.edu.cn.

PMID: 24270065
DOI: 10.1016/j.ymeth.2013.11.004

Abstract

The functionalized DNA has been widely developed and played a more and more important role in life science and material science during last decades. Therefore, methods to effectively endue DNA new functions by modifying DNA have been developed quickly. In this review, we will give an introduction in the methods for covalent terminal functionalization of DNA, including solid-phase functionalization and solution coupling functionalization, mainly on the technical aspect. The application of these functionalized DNA will be also introduced.

Keywords: DNA; Solid-phase functionalization; Solution coupling functionalization; Terminal functionalization.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Amides / chemical synthesis
Amides / chemistry
Catalysis
Click Chemistry
Copper / chemistry
DNA / chemical synthesis*
Nanostructures / chemistry
Phosphoric Acids / chemistry
Solid-Phase Synthesis Techniques
Solutions

Substances

Amides
Phosphoric Acids
Solutions
Copper
DNA
phosphoramidic acid