Acetylcholine operated ion channel and alpha-bungarotoxin binding site in a human neuroblastoma cell line reside on different molecules

Biochem Biophys Res Commun. 1986 Jun 30;137(3):1141-7. doi: 10.1016/0006-291x(86)90344-x.

Abstract

In neurons, alpha-bungarotoxin is often associated with nicotinic receptor but does not always block the acetylcholine operated channel. In a human neuroblastoma cell line, IMR 32, we have demonstrated a large number of alpha-Bungarotoxin binding sites (2640 per cell in non differentiated cells and 4660 per cell in differentiated cells) in presence of 0 to 4 Acetylcholine activated-channels per cell. This neuronal cell line promises to be an useful model for the study of structure and function of the alpha-Bungarotoxin binding site not related to the nicotinic receptor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcholine / metabolism
  • Binding Sites
  • Bungarotoxins / metabolism*
  • Cell Line
  • Electric Conductivity
  • Humans
  • Ion Channels / metabolism*
  • Macromolecular Substances
  • Neuroblastoma
  • Receptors, Nicotinic / metabolism*

Substances

  • Bungarotoxins
  • Ion Channels
  • Macromolecular Substances
  • Receptors, Nicotinic
  • Acetylcholine