A sensitive plasmonic chirality-based aptasensor for the detection of bisphenol A (BPA) was developed. Asymmetric plasmonic nanoparticle dimers were produced by the hybridization of a BPA aptamer and its complementary sequence modified nanoparticles. Under different concentrations of BPA, the intensity of the chiral signal was varied. A low limit of detection of 0.008 ng/mL was obtained in the range 0.02-5 ng/mL.