Abstract
Mycobacterium tuberculosis (Mtb) restrains immune responses well enough to escape eradication but elicits enough immunopathology to ensure its transmission. Here we provide evidence that this host-pathogen relationship is regulated in part by a cytosolic, membrane-associated protein with a unique structural fold, encoded by the Mtb gene rv0431. The protein acts by regulating the quantity of Mtb-derived membrane vesicles bearing Toll-like receptor 2 ligands, including the lipoproteins LpqH and SodC. We propose that rv0431 be named "vesiculogenesis and immune response regulator."
Keywords:
TLR2; macrophages.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bacterial Proteins / chemistry*
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Bacterial Proteins / metabolism
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Female
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Host-Pathogen Interactions
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Immunomodulation / genetics
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Immunomodulation / physiology*
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Lipoproteins / metabolism*
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Macrophages
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Magnetic Resonance Spectroscopy
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Membrane Proteins / chemistry*
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Membrane Proteins / metabolism
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Microscopy, Electron, Scanning
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Models, Molecular*
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Mycobacterium tuberculosis / genetics*
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Mycobacterium tuberculosis / immunology*
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Protein Folding
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Toll-Like Receptor 2 / agonists
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Toll-Like Receptor 2 / genetics
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Transport Vesicles / metabolism
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Transport Vesicles / physiology*
Substances
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Bacterial Proteins
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Lipoproteins
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Membrane Proteins
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Toll-Like Receptor 2