Estimates of recombination frequency among enzyme loci of pitch pine revealed two new linkages, Mdh3:Pgm2 (θ=0.01) and Pep1:Mdh4 (θ=0.38), and confirmed two previously established linkages. Tighter linkage (θ=0.30) was ruled out for nearly all gene pairs examined. In general, the Bayesian approach used in this study to test for linkage performed better than alternative methods.