Gold staining in cellulose acetate membranes

Clin Chim Acta. 1986 Jun 15;157(2):167-74. doi: 10.1016/0009-8981(86)90222-6.

Abstract

A novel method for revealing proteins after electrophoresis on cellulose acetate is described, based on adsorption of mixed gold-Tween 20 micelles onto TCA-fixed protein bands. The sensitivity is 2-300 times higher than conventional Coomassie Blue staining and ca. 10 fold higher than silver coloring, detecting barely 1 ng protein/mm2 gel. Acidic and ammoniacal silver dyeing, based on ionic silver, perform very poorly on cellulose matrices, while a new variant, colloidal silver, stains protein bands on a clear background, but with a sensitivity ca. 10 times lower than micellar gold. Urines, cerebrospinal fluid and other biological liquids can be revealed without resorting to a concentration step prior to electrophoresis. The method is simple to perform and does not require any destaining step.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blood Proteins / analysis
  • Cerebrospinal Fluid Proteins / analysis
  • Electrophoresis, Cellulose Acetate
  • Gold*
  • Humans
  • Proteins / analysis*
  • Proteinuria / urine
  • Rosaniline Dyes
  • Serum Albumin / analysis
  • Silver
  • Staining and Labeling*

Substances

  • Blood Proteins
  • Cerebrospinal Fluid Proteins
  • Proteins
  • Rosaniline Dyes
  • Serum Albumin
  • Silver
  • Coomassie brilliant blue R
  • Gold