We newly fabricated and characterized the double layered nanoporous Pt (NPt) thin film on silicon substrate for enzyme-free glucose sensing applications. The surface morphologies of the fabricated NPt film were characterized using field emission scanning electron microscopy (FESEM). The fabricated NPt films exhibited highly dense nanopores with irregularly arranged grain boundaries and thicknesses of 0.77 microm, which were highly affected by the structural geometries of the bottom layer. The electrochemical properties of the double layered NPt films were tested in 1 M sulfuric acid and phosphate buffered saline (PBS, pH 7.4) with different concentrations of glucose. The NPt film on silicon substrate exhibited extremely high electrochemical roughness factor (RF) of 901.35 (mean values) and sensitivities of 33.34 microA mM(-1) cm(-2) in glucose concentrations up to 16 mM.