We studied the suitability of Panchgavya (five products of cow), new organic amendment, application on seed germination, plant growth, and soil health. After characterization, Panchgavya was mixed with water to form different concentration and was tested for seed germination, germination index, and root and shoot growth of different seedlings. Four percent solution of Panchgavya was applied to different plants to test its efficacy. Panchgavya and other two organic amendments were incorporated in soil to test the change of soil chemical and microbiological parameters. Panchgavya contained higher nutrients as compared to farm yard manure (FYM) and vermicompost. Its application on different seeds has positively influenced germination percentage, germination index, root and shoot length, and fresh and dry weight of the seedling. Water-soluble macronutrients including pH and metal were positively and negatively correlated with the growth parameters, respectively. Four percent solution of Panchgavya application on some plants showed superiority in terms of plant height and chlorophyll content. Panchgavya-applied soil had higher values of macro and micronutrients (zinc, copper, and manganese), microbial activity as compared to FYM, and vermicompost applied soils. Application of Panchgavya can be gainfully used as an alternative organic supplement in agriculture.