[Authenticate of Atractylodes macrocephala seed by amplification refractory mutation system]

Liang Cao; Chao Jiang; Hua-Sheng Peng; Min Chen; Yuan Yuan

[Authenticate of Atractylodes macrocephala seed by amplification refractory mutation system]

Zhongguo Zhong Yao Za Zhi. 2013 Aug;38(16):2567-70.

[Article in Chinese]

Authors

Liang Cao¹, Chao Jiang, Hua-Sheng Peng, Min Chen, Yuan Yuan

Affiliation

¹ National Resource Center for Chinese Materia Medica, China Academy of Chinese Medicinal Sciences, Beijing 100700, China.

PMID: 24228563

Abstract

Objective: To design specific primers and authenticate Atractylodes macrocephala from Atractylodes lancea and A. chinensis.

Method: SNPs in the psbA-trnH sequences of Atractylodes were found by ClustulW program and Bioedit software. Primers for authentic A. macrocephala is designed according to the SNP site, and ITS sequence universal primers plus to the authentic primer to construct a multi-PCR reaction system, and then optimized the PCR reaction system.

Result: 172 bp band special for A. macrocephala were found using multi-PCR reaction.

Conclusion: The multi-PCR reaction system could be applied to identify A. macrocephala seed.

Publication types

English Abstract

MeSH terms

Atractylodes / genetics*
DNA Primers / genetics
Mutation*
Polymerase Chain Reaction / methods*
Quality Control
Seeds / genetics*

Substances

DNA Primers