Direct gene transfer to protoplasts of Arabidopsis thaliana

H Karesch; R Bilang; O Mittelsten Scheid; I Potrykus

doi:10.1007/BF00232334

Direct gene transfer to protoplasts of Arabidopsis thaliana

Plant Cell Rep. 1991 Feb;9(10):571-4. doi: 10.1007/BF00232334.

Authors

H Karesch¹, R Bilang, O Mittelsten Scheid, I Potrykus

Affiliation

¹ Institute of Plant Sciences, Swiss Federal Institute of Technology (ETH), ETH-Zentrum, CH-8092, Zürich, Switzerland.

PMID: 24220714
DOI: 10.1007/BF00232334

Abstract

We performed a series of direct gene transfer experiments with protoplasts of Arabidopsis thaliana ecotype Zürich. An average of more than 100 transformants were selected per 10(6)6 treated protoplasts. Stable transformation was confirmed by integration of the marker gene into high molecular weight DNA and by its genetic transmission to subsequent offspring generations.