Identification of a novel N4BP1-like gene from grass carp (Ctenopharyngodon idella) in response to GCRV infection

Jia Cai; Lin Yang; Bei Wang; Yucong Huang; Jufen Tang; Yishan Lu; Zaohe Wu; Jichang Jian

doi:10.1016/j.fsi.2013.11.003

Identification of a novel N4BP1-like gene from grass carp (Ctenopharyngodon idella) in response to GCRV infection

Fish Shellfish Immunol. 2014 Jan;36(1):223-8. doi: 10.1016/j.fsi.2013.11.003. Epub 2013 Nov 9.

Authors

Jia Cai¹, Lin Yang¹, Bei Wang¹, Yucong Huang¹, Jufen Tang¹, Yishan Lu¹, Zaohe Wu², Jichang Jian³

Affiliations

¹ College of Fishery, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang 524088, China.
² Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang 524088, China; Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
³ College of Fishery, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang 524088, China. Electronic address: jianjc@gmail.com.

PMID: 24220004
DOI: 10.1016/j.fsi.2013.11.003

Abstract

Nedd4 binding protein 1 (N4BP1) has been identified as an interacting protein and a substrate of Nedd4 E3 ligase. However, the report about N4BP1's function is limit. In this study, a novel N4BP1 gene (CiN4BP1) was cloned from grass carp (Ctenopharyngodon idella). The full-length cDNA sequence of CiN4BP1 (3022 bp) included an open reading frame (ORF) of 2565 bp, which encoded a putative peptides of 854 amino acids containing one KH domain and one NYN domain. It was close homology (47% identify) to Oryzias latipes N4BP1. And mRNA expression of CiN4BP1 gene showed relatively high level in skin, gill, head kidney and spleen. After grass carp reovirus (GCRV) infection, CiN4BP1 was up-regulated in vivo and in vitro. Furthermore, overexpression of CiN4BP1 in CIK cells inhibited viral gene transcription. These data indicated that CiN4BP1 might play an important role in immune response to viral invasion.

Keywords: Ctenopharyngodon idella; Grass carp reovirus (GCRV); N4BP1; Viral gene; Viral invasion.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Carps*
Cell Line
Cloning, Molecular
Fish Diseases / immunology
Fish Diseases / virology*
Fish Proteins / genetics
Fish Proteins / immunology*
Molecular Sequence Data
Phylogeny*
RNA / chemistry
RNA / genetics
Reoviridae / immunology*
Reoviridae Infections / immunology
Reoviridae Infections / veterinary*
Reverse Transcriptase Polymerase Chain Reaction / veterinary
Sequence Alignment
Sequence Analysis, DNA

Substances

Fish Proteins
RNA

Associated data

GENBANK/KF590031