Inhibition of α-glucosidase and glucose intestinal absorption by Thymelaea hirsuta fractions

Abstract

Background: Thymelaea hirsuta (L.) Endl. (Thymelaeaceae) is a medicinal plant used in Morocco to treat diabetes. In previous studies T. hirsuta has shown a potent antihyperglycemic effect. Our aim was to study the effect of the plant on α-glucosidase inhibition and intestinal glucose absorption.

Methods: Five fractions of T. hirsuta were tested, in vitro, in vivo and, in situ, to elucidate the inhibition of α-glucosidase and intestinal glucose uptake.

Results: The fractions induced, in vitro, a significant inhibition of α-glucosidase. The ethyl acetate fraction (EATh) had high activity and its inhibition mode was non-competitive. The EATh at 50 and 100 mg/kg doses, decreased significantly, in vivo, the postprandial hyperglycemia after sucrose loading in normal and diabetic mice. Moreover, 50 mg/kg of EATh significantly decreased intestinal glucose uptake, in situ, in rats.

Conclusion: The antihyperglycemic effect of T. hirsuta can be explained, in part, by the inhibition of intestinal α-glucosidase and intestinal glucose absorption.

Keywords: Thymelaea hirsuta; diabetes; intestinal glucose absorption; streptozotocin; α-glucosidase inhibitor; 糖尿病，肠道葡萄糖吸收，链脲霉素，毛花瑞香，α-糖甙酶抑制作用.