Developments in purification methods for obtaining and evaluation of collagen derived endogenous angioinhibitors

Protein Expr Purif. 2014 Feb:94:46-52. doi: 10.1016/j.pep.2013.10.021. Epub 2013 Nov 9.

Abstract

Collagen constitutes one of the vital components of the basement membrane scaffolds. Non-collagenous domains (NC1) derived from collagens exhibit potent anti-angiogenic properties, thus attaining significance in regulation of angiogenesis promoted diseases. Individual NC1 domains essential for anti-angiogenic evaluations are generally obtained through purification of individual non-collagenous domains, which have undergone steady developments for enhancing the yields, purpose of biological evaluations and solubility based on the nature of different NC1 domains. This review focuses on the method developments in obtaining biologically active NC1 domains and for specific evaluations in different scenarios.

Keywords: Anti-angiogenesis; Different expression systems; Different purification methods; Extra cellular matrix (ECM); Non-collagenous domains (NC1); Type IV collagen.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Angiogenesis Inhibitors / administration & dosage
  • Angiogenesis Inhibitors / chemistry
  • Angiogenesis Inhibitors / genetics
  • Basement Membrane / chemistry
  • Basement Membrane / metabolism*
  • Collagen Type IV / chemistry
  • Collagen Type IV / genetics
  • Collagen Type IV / isolation & purification*
  • Extracellular Matrix / chemistry
  • Extracellular Matrix / genetics
  • Gene Expression Regulation
  • Humans
  • Protein Structure, Tertiary*

Substances

  • Angiogenesis Inhibitors
  • Collagen Type IV