Quantum-dot-based immunochromatographic assay for total IgE in human serum

PLoS One. 2013 Oct 30;8(10):e77485. doi: 10.1371/journal.pone.0077485. eCollection 2013.

Abstract

To rapidly quantify total immunoglobulin E levels in human serum, we developed a novel quantum-dot-based immunochromatographic assay that employs digital recording of fluorescence. It can detect IgE levels of 5-1000 kU/L, with a coefficient of variation ranging from 2.0 to 9.5%. The assay can be processed in 10 min. The developed assay was tested on 95 serum samples. The correlation coefficient between the IgE values obtained by the proposed assay and those obtained by a commercial ELISA kit was 0.9884. Our assay thus shows promise as a new diagnostic tool for IgE detection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / chemistry
  • Antibodies, Monoclonal / isolation & purification
  • Calibration
  • Chromatography, Affinity / methods*
  • Goats
  • Humans
  • Hypersensitivity / blood*
  • Hypersensitivity / diagnosis
  • Immunoglobulin E / blood*
  • Mice
  • Quantum Dots / chemistry*
  • Reproducibility of Results
  • Sensitivity and Specificity

Substances

  • Antibodies, Monoclonal
  • Immunoglobulin E

Grants and funding

This work was supported by the Ministry of Education and Science of the Russian Federation (State Target Program «Research and scientific-pedagogical personnel of innovative Russia in 2009–2013, contract No. 8284). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.