The white-rot fungus pleurotus ostreatus transformant overproduced intracellular cAMP and laccase

Biosci Biotechnol Biochem. 2013;77(11):2309-11. doi: 10.1271/bbb.130470. Epub 2013 Nov 7.

Abstract

Transformation of Pleurotus ostreatus PC9 with the mutated heterotrimeric G protein alpha subunit (Gα) gene resulted in higher laccase (Lac) activity and intracellular cyclic adenosine monophosphate (cAMP) concentrations as compared to those in wild-type PC9. The transformant also exhibited higher Lac activity than the wild type when cultured in a medium containing known Lac inducers CuSO4 and ferulic acid.

MeSH terms

  • Copper Sulfate / pharmacology
  • Coumaric Acids / pharmacology
  • Culture Media
  • Cyclic AMP / biosynthesis*
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism
  • GTP-Binding Protein alpha Subunits / genetics*
  • GTP-Binding Protein alpha Subunits / metabolism
  • Gene Expression Regulation, Fungal*
  • Laccase / biosynthesis
  • Laccase / genetics*
  • Mutation
  • Pleurotus / drug effects
  • Pleurotus / enzymology
  • Pleurotus / genetics*
  • Transformation, Genetic
  • Transgenes

Substances

  • Coumaric Acids
  • Culture Media
  • Fungal Proteins
  • GTP-Binding Protein alpha Subunits
  • ferulic acid
  • Cyclic AMP
  • Laccase
  • Copper Sulfate