Abstract
Transformation of Pleurotus ostreatus PC9 with the mutated heterotrimeric G protein alpha subunit (Gα) gene resulted in higher laccase (Lac) activity and intracellular cyclic adenosine monophosphate (cAMP) concentrations as compared to those in wild-type PC9. The transformant also exhibited higher Lac activity than the wild type when cultured in a medium containing known Lac inducers CuSO4 and ferulic acid.
MeSH terms
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Copper Sulfate / pharmacology
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Coumaric Acids / pharmacology
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Culture Media
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Cyclic AMP / biosynthesis*
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Fungal Proteins / genetics*
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Fungal Proteins / metabolism
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GTP-Binding Protein alpha Subunits / genetics*
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GTP-Binding Protein alpha Subunits / metabolism
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Gene Expression Regulation, Fungal*
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Laccase / biosynthesis
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Laccase / genetics*
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Mutation
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Pleurotus / drug effects
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Pleurotus / enzymology
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Pleurotus / genetics*
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Transformation, Genetic
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Transgenes
Substances
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Coumaric Acids
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Culture Media
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Fungal Proteins
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GTP-Binding Protein alpha Subunits
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ferulic acid
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Cyclic AMP
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Laccase
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Copper Sulfate