To explore the effect of Xiaoer Fuxie Waifu powder on gastrointestinal dynamics. In the study, the charcoal powder propelling test was used to observe the effect Xiaoer Fuxie Waifu powder on the intestinal motility of normal mice and deficient diarrheal mice. The results showed that Xiaoer Fuxie Waifu powder had no obvious inhibitory effect on the powder propelling in normal mice, but a remarkable inhibitory effect on deficient diarrheal mice. Afterwards, diarrhea index was used to observe the effect on deficient diarrheal mice. The results showed a good anti-diarrhea effect in deficient diarrheal mice induced by folium sennae. According to the subsequent isolated intestines smooth muscle test, Xiaoer Fuxie Waifu powder showed a competitive antagonism on muscular tension caused by acetyl choline and barium chloride, as well as a certain synergistic action with atropine. Finally, the method of radio-immunity was used to determine the contents of SP and VIP in small intestine of mice, showing that SP and VIP in small intestine of mice were decreased and the motility of intestine was inhibited. In conclusion, both in vitro and in vivo experiments indicate that Xiaoer Fuxie Waifu powder is a good traditional Chinese gastrointestinal dynamic medicine for external application, with an anti-diarrhea effect on diarrhea mice induced by spleen deficiency and diarrhea.