Linkage analysis of er-1, a recessive Pisum sativum gene for resistance to powdery mildew fungus (Erysiphe pisi D.C.)

G M Timmerman; T J Frew; N F Weeden; A L Miller; D S Goulden

doi:10.1007/BF00220815

Linkage analysis of er-1, a recessive Pisum sativum gene for resistance to powdery mildew fungus (Erysiphe pisi D.C.)

Theor Appl Genet. 1994 Sep;88(8):1050-5. doi: 10.1007/BF00220815.

Authors

G M Timmerman¹, T J Frew, N F Weeden, A L Miller, D S Goulden

Affiliation

¹ New Zealand Institute for Crop & Food Research Ltd, Private Bag 4704, Christchurch, New Zealand.

PMID: 24186261
DOI: 10.1007/BF00220815

Abstract

Linkage analysis was used to determine the genetic map location of er-1, a recessive gene conditioning resistance to powdery mildew, on the Pisum sativum genome. Genetic linkage was demonstrated between er-1 and linkage group 6 markers after analyzing the progeny of two crosses, an F2 population and a set of recombinant inbred lines. The classes of genetic markers surrounding er-1 include RFLP, RAPD and allozyme markers as well as the morphological marker Gty. A RAPD marker tightly linked to er-1 was identified by bulked segregant analysis. After DNA sequence characterization, specific PCR primers were designed to convert this RAPD marker into a sequence characterized amplified region (SCAR).