Characterization of three Chlorella sorokiniana strains in anaerobic digested effluent from cattle manure

Bioresour Technol. 2013 Dec:150:377-86. doi: 10.1016/j.biortech.2013.10.032. Epub 2013 Oct 18.

Abstract

Chlorella sorokiniana CS-01, UTEX 1230 and UTEX 2714 were maintained in 10% anaerobic digester effluent (ADE) from cattle manure digestion and compared with algal cultivation in Bold's Basal Medium (BBM). Biomass of CS-01 and UTEX 1230 in ADE produced similar or greater than 280mg/L after 21days in BBM, however, UTEX 2714 growth in ADE was suppressed by more than 50% demonstrating a significant species bias to synthetic compared to organic waste-based media. The highest accumulation of protein and starch was exhibited in UTEX 1230 in ADE yielding 34% and 23% ash free dry weight (AFDW), respectively, though fatty acid methyl ester total lipid measured less than 12% AFDW. Results suggest that biomass from UTEX 1230 in ADE may serve as a candidate alga and growth system combination sustainable for animal feed production considering high yields of protein, starch and low lipid accumulation.

Keywords: Anaerobic digester effluent; Biomass; Chlorella sorokiniana; Nutrient removal; Starch.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Ammonium Compounds / metabolism
  • Anaerobiosis
  • Animals
  • Biomass
  • Cattle
  • Chlorella / drug effects
  • Chlorella / growth & development*
  • Culture Media / pharmacology
  • Esters / analysis
  • Fatty Acids / analysis
  • Manure*
  • Nitrates / metabolism
  • Nitrites / metabolism
  • Nitrogen / metabolism
  • Phosphorus / metabolism
  • Starch / metabolism
  • Waste Disposal, Fluid*

Substances

  • Ammonium Compounds
  • Culture Media
  • Esters
  • Fatty Acids
  • Manure
  • Nitrates
  • Nitrites
  • Phosphorus
  • Starch
  • Nitrogen