Nitrous oxide reduction genetic potential from the microbial community of an intermittently aerated partial nitritation SBR treating mature landfill leachate

Abstract

This study investigates the microbial community dynamics in an intermittently aerated partial nitritation (PN) SBR treating landfill leachate, with emphasis to the nosZ encoding gene. PN was successfully achieved and high effluent stability and suitability for a later anammox reactor was ensured. Anoxic feedings allowed denitrifying activity in the reactor. The influent composition influenced the mixed liquor suspended solids concentration leading to variations of specific operational rates. The bacterial community was low diverse due to the stringent conditions in the reactor, and was mostly enriched by members of Betaproteobacteria and Bacteroidetes as determined by 16S rRNA sequencing from excised DGGE melting types. The qPCR analysis for nitrogen cycle-related enzymes (amoA, nirS, nirK and nosZ) demonstrated high amoA enrichment but being nirS the most relatively abundant gene. nosZ was also enriched from the seed sludge. Linear correlation was found mostly between nirS and the organic specific rates. Finally, Bacteroidetes sequenced in this study by 16S rRNA DGGE were not sequenced for nosZ DGGE, indicating that not all denitrifiers deal with complete denitrification. However, nosZ encoding gene bacteria was found during the whole experiment indicating the genetic potential to reduce N2O.

Keywords: AOB; BOD; COD; Denitrification; EUB; Eubacteria; FA; FISH; FNA; Fluorescence in situ hybridization; MFC; MLVSS; Microbial community; N(2)O; NOB; PCR-DGGE; PN-SBR; Partial nitritation; TN; TSS; VSS; ammonium oxidation enzyme; ammonium oxidizing bacteria; amoA; biochemical oxygen demand; chemical oxygen demand; free ammonia; free nitrous acid; microbial fuel cell; mixed liquor volatile suspended solids; nirK; nirS; nitrate reduction enzyme; nitrite oxidation bacteria; nitrite oxidation enzyme; nitrite reduction enzyme (K); nitrite reduction enzyme (S); nitrous oxide reduction enzyme; nor; nosZ; nox; partial nitritation sequencing batch reactor; polymerase chain reaction-denaturing gradient gel electrophoresis; qPCR; quantitative polymerase chain reaction; sALR; sNPR; sNRR; sOLR; sORR; specific ammonium loading rate; specific nitrite production rate; specific nitrogen removal rate; specific organic loading rate; specific organic removal rate; total nitrogen; total suspended solids; volatile suspended solids.