Regulation of transcription of the Saccharomyces cerevisiae CYC1 gene: Identification of a DNA region involved in heme control

R Gudenus; A Spence; A Hartig; M Smith; H Ruis

doi:10.1007/BF00405431

Regulation of transcription of the Saccharomyces cerevisiae CYC1 gene: Identification of a DNA region involved in heme control

Curr Genet. 1984 Jan;8(1):45-8. doi: 10.1007/BF00405431.

Authors

R Gudenus¹, A Spence, A Hartig, M Smith, H Ruis

Affiliation

¹ Institut für Allgemeine Biochemie der Universität Wien, Ludwig Boltzmann-Forschungsstelle für Biochemie, Währinger Str. 38, A-1090, Wien, Austria.

PMID: 24177529
DOI: 10.1007/BF00405431

Abstract

A Saccharomyces cerevisiae mutant (hem1 cycl-1) was transformed with plasmids bearing a chromosomal centromer (CEN3) and a 2 μm DNA replication origin. In one of the plasmids a functional CYC1 gene was present, in a second plasmid an XhoI fragment located between bases -245 and -678 upstream from the translation initiation codon had been deleted, in a third plasmid this region had been inverted. Results of hybridization experiments carried out with mRNA isolated from heme-deficient and heme-containing transformants indicated that heme controls transcription of the CYC1 gene and that DNA sequences located within the upstream XhoI fragment are involved in activation of the gene by heme.