Characterization of N-demethyllincosamide methyltransferases LmbJ and CcbJ

Chembiochem. 2013 Nov 25;14(17):2259-62. doi: 10.1002/cbic.201300389. Epub 2013 Oct 25.

Abstract

Chemical diversity: Two SAM-dependent N-methyltransferases-LmbJ from the biosynthesis of the antibiotic lincomycin and CcbJ from celesticetin biosynthesis-have been characterized and compared. Both tested enzymes form multimers and are able to utilize N-demethyllincomycin, the natural substrate of LmbJ, with comparable efficiency.

Keywords: N-methyltransferases; Streptomyces; antibiotics; lincomycin; natural products.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / biosynthesis*
  • Anti-Bacterial Agents / chemistry
  • Biocatalysis*
  • Lincomycin / biosynthesis*
  • Lincomycin / chemistry
  • Lincosamides / biosynthesis*
  • Lincosamides / chemistry
  • Methyltransferases / chemistry
  • Methyltransferases / metabolism*
  • Molecular Conformation
  • Substrate Specificity

Substances

  • Anti-Bacterial Agents
  • Lincosamides
  • celesticetin A
  • Lincomycin
  • Methyltransferases