Detection of microRNA maturation using unmodified pre-microRNA and branched rolling circle amplification

Methods Mol Biol. 2014:1095:109-19. doi: 10.1007/978-1-62703-703-7_9.

Abstract

The ever-increasing number of different miRNAs and their association with a vast number of cellular dysfunctions and diseases have initiated several groups to investigate miRNA maturation, which ultimately leads to down regulation of a target messenger RNA (mRNA) and its downstream product. A rapid, convenient, and reliable assay to detect the Dicer-mediated miRNA-maturation step may facilitate research in this field. Here we describe the in vitro detection of the Dicer-mediated miRNA maturation step using unmodified pre-miRNA and branched rolling circle amplification.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Replication*
  • DNA, Single-Stranded / metabolism
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Nucleic Acid Amplification Techniques / methods*
  • Phosphorylation
  • RNA Cleavage
  • RNA Precursors / genetics
  • RNA Precursors / metabolism*
  • Ribonuclease III / metabolism
  • Transcription, Genetic

Substances

  • DNA, Single-Stranded
  • MicroRNAs
  • RNA Precursors
  • Ribonuclease III