Abstract
We produced three highly purified recombinant antigens rLipL32, rLipL41, and rLigA-Rep (leptospiral immunoglobulin-like A repeat region) for the detection of Leptospira-specific antibodies in an enzyme-linked immunosorbent assay (ELISA). The performance of these recombinant antigens was evaluated using 121 human sera. Among them, 63 sera were microscopic agglutination test (MAT)-confirmed positive sera from febrile patients in Peru, 22 sera were indigenous MAT-negative febrile patient sera, and 36 sera were from patients with other febrile diseases from Southeast Asia, where leptospirosis is also endemic. Combining the results of immunoglobulin M (IgM) and IgG detection from these three antigens, the overall sensitivity is close to 90% based on the MAT. These results suggest that an ELISA using multiple recombinant antigens may be used as an alternative method for the detection of Leptospira-specific antibodies.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Agglutination Tests
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Antibodies, Bacterial / blood*
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Antigens, Bacterial / genetics
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Antigens, Bacterial / immunology*
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Antigens, Bacterial / metabolism
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Bacterial Outer Membrane Proteins / genetics
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Bacterial Outer Membrane Proteins / metabolism
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Enzyme-Linked Immunosorbent Assay / methods*
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Humans
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Immunoglobulin G / blood
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Immunoglobulin M / blood
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Leptospira / immunology*
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Leptospira / isolation & purification
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Leptospirosis / diagnosis*
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Leptospirosis / microbiology
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Lipoproteins / genetics
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Lipoproteins / metabolism
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Peru
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Recombinant Proteins / immunology
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Species Specificity
Substances
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Antibodies, Bacterial
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Antigens, Bacterial
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Bacterial Outer Membrane Proteins
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Bacterial Proteins
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Immunoglobulin G
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Immunoglobulin M
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LipL32 protein, Leptospira
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LipL41 protein, Leptospira interrogans
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Lipoproteins
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Recombinant Proteins