Inheritance of foreign genes in transgenic bean (Phaseolus vulgaris L.) co-transformed via particle bombardment

F J Aragão; L M Barros; A C Brasileiro; S G Ribeiro; F D Smith; J C Sanford; J C Faria; E L Rech

doi:10.1007/BF00225739

Inheritance of foreign genes in transgenic bean (Phaseolus vulgaris L.) co-transformed via particle bombardment

Theor Appl Genet. 1996 Jul;93(1-2):142-50. doi: 10.1007/BF00225739.

Authors

F J Aragão¹, L M Barros, A C Brasileiro, S G Ribeiro, F D Smith, J C Sanford, J C Faria, E L Rech

Affiliation

¹ Centro Nacional de Pesquisa de Recursos Genéticos e Biotecnologia, EMBRAPA, P.O. Box 02372, 70849-970, Brasília, DF, Brazil.

PMID: 24162211
DOI: 10.1007/BF00225739

Abstract

Exploiting the biolistic process we have generated stable transgenic bean (Phaseolus vulgaris L.) plants with unlinked and linked foreign genes. Co-transformation was conducted using plasmid constructions containing a fusion of the gus and neo genes, which were co-introduced with the methionine-rich 2S albumin gene isolated from the Brazil nut and the antisense sequence of AC1, AC2, AC3 and BC1 genes from the bean golden mosaic geminivirus. The results revealed a co-transformation frequency ranging from 40% to 50% when using unlinked genes and 100% for linked genes. The introduced foreign genes were inherited in a Mendelian fashion in most of the transgenic bean lines. PCR and Southern blot hybridization confirmed the integration of the foreign genes in the plant genome.