In vivo three dimensional dual wavelength photoacoustic tomography imaging of the far red fluorescent protein E2-Crimson expressed in adult zebrafish

Mengyang Liu; Nicole Schmitner; Michelle G Sandrian; Behrooz Zabihian; Boris Hermann; Willi Salvenmoser; Dirk Meyer; Wolfgang Drexler

doi:10.1364/BOE.4.001846

In vivo three dimensional dual wavelength photoacoustic tomography imaging of the far red fluorescent protein E2-Crimson expressed in adult zebrafish

Biomed Opt Express. 2013 Aug 29;4(10):1846-55. doi: 10.1364/BOE.4.001846. eCollection 2013.

Authors

Mengyang Liu¹, Nicole Schmitner, Michelle G Sandrian, Behrooz Zabihian, Boris Hermann, Willi Salvenmoser, Dirk Meyer, Wolfgang Drexler

Affiliation

¹ Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Währinger Gürtel 18-20, AKH 4L, 1090 Vienna, Austria.

Abstract

For the first time the far red fluorescent protein (FP) E2-Crimson genetically expressed in the exocrine pancreas of adult zebrafish has been non-invasively mapped in 3D in vivo using photoacoustic tomography (PAT). The distribution of E2-Crimson in the exocrine pancreas acquired by PAT was confirmed using epifluorescence imaging and histology, with optical coherence tomography (OCT) providing complementary structural information. This work demonstrates the depth advantage of PAT to resolve FP in an animal model and establishes the value of E2-Crimson for PAT studies of transgenic models, laying the foundation for future longitudinal studies of the zebrafish as a model of diseases affecting inner organs.

Keywords: (170.3880) Medical and biological imaging; (170.5120) Photoacoustic imaging; (170.6280) Spectroscopy, fluorescence and luminescence; (170.6960) Tomography.