Abstract
Stabilized Wittig olefination holds great potential as a bioconjugation reaction. We demonstrate that the reaction of stabilized phosphorus ylides (or phosphonium salts) with aryl aldehydes is sufficiently robust to be used for live cell affinity isolation and fluorescence tagging of a protein, FKBP12.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aldehydes / chemistry
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Alkenes / chemistry*
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Fluorescent Dyes / chemistry
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HeLa Cells
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Humans
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Microscopy, Fluorescence
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Phosphorus / chemistry
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Tacrolimus Binding Protein 1A / chemistry*
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Tacrolimus Binding Protein 1A / metabolism
Substances
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Aldehydes
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Alkenes
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Fluorescent Dyes
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Phosphorus
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Tacrolimus Binding Protein 1A