Stabilized Wittig olefination for bioconjugation

Chem Commun (Camb). 2013 Dec 11;49(95):11188-90. doi: 10.1039/c3cc45961f. Epub 2013 Oct 23.

Abstract

Stabilized Wittig olefination holds great potential as a bioconjugation reaction. We demonstrate that the reaction of stabilized phosphorus ylides (or phosphonium salts) with aryl aldehydes is sufficiently robust to be used for live cell affinity isolation and fluorescence tagging of a protein, FKBP12.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aldehydes / chemistry
  • Alkenes / chemistry*
  • Fluorescent Dyes / chemistry
  • HeLa Cells
  • Humans
  • Microscopy, Fluorescence
  • Phosphorus / chemistry
  • Tacrolimus Binding Protein 1A / chemistry*
  • Tacrolimus Binding Protein 1A / metabolism

Substances

  • Aldehydes
  • Alkenes
  • Fluorescent Dyes
  • Phosphorus
  • Tacrolimus Binding Protein 1A