This study examined the relationship between indicators of acculturation and weight loss strategies among Latinas. Latinas residing in the United States longer had more experience with various weight loss strategies (r = 0.24, p = 0.05). Controlling for demographic factors and BMI, years of U.S. residence was related to whether increased physical activity (OR 1.18, 95% CI 1.01-1.39, p = 0.04) and reduced portion sizes (OR 1.39, 95% CI 1.02-1.91, p = 0.03) were used as strategies for weight loss. More English spoken at home was associated with use of reducing calories as a strategy for weight loss (OR 4.13, 95% CI 1.06-16.09, p = 0.04). Regardless of acculturation level, less empirically supported methods such as using herbal products and a girdle were more prevalent than commonly recommended methods such as using meal replacement products and commercial weight loss programs. Behavioral weight loss interventions for Latinas should consider acculturation to more effectively target subgroups, address cultural practices, and teach lifestyle-appropriate strategies.