Applied orthogonal experiment design for the optimum microwave-assisted extraction conditions of polysaccharides from Rhodiolae Radix

Fenglin Li; Furen Xiao; Jingli Gong; Tian Yu

doi:10.4314/ajtcam.v10i4.29

Applied orthogonal experiment design for the optimum microwave-assisted extraction conditions of polysaccharides from Rhodiolae Radix

Afr J Tradit Complement Altern Med. 2013 May 16;10(4):179-85. doi: 10.4314/ajtcam.v10i4.29. eCollection 2013.

Authors

Fenglin Li¹, Furen Xiao, Jingli Gong, Tian Yu

Affiliation

¹ Jilin Agricultural Science and Technology College, Jilin, 132101, China ; Brewing Technology Innovation Center of Jilin Province, Jilin, 132101, China ; Key Laboratory of Metastable Materials Science and Technology, College of Materials Science and Engineering, Yanshan University, Qinghuangdao, 066004, China.

Abstract

An experiment on polysaccharides from Rhodiolae Radix (PRR) extraction was carried out using microwave-assisted extraction (MAE) method with an objective to establishing the optimum MAE conditions of PRR. Single factor experiments were performed to determine the appropriate range of extraction conditions, and the optimum conditions were obtained using orthogonal experiment design. The results showed that the optimum MAE conditions of PRR were as follows: solid-liquid ratio of 1:45 g/mL, irradiation power of 480 W, and irradiation time of 8 min, while extraction yield of PRR was 3.24 %.

Keywords: Rhodiolae Radix; microwave-assisted extraction; orthogonal experiment design; polysaccharides.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Drugs, Chinese Herbal / chemistry*
Microwaves
Plant Roots / chemistry*
Polysaccharides / isolation & purification*
Rhodiola / chemistry*

Substances

Drugs, Chinese Herbal
Polysaccharides