Abstract
The purpose of this manuscript was to study the regulation effects of β-elemene combined with radiotherapy on three different gene expressions in lung adenocarcinoma A549 cell. mTOR gene, HIF-1α gene, Survivin gene were included in the gene group. Cell culture and RT-PCR were applied to finish this research. Hypoxia Control group, Hypoxia β-elemene group, Hypoxia β-elemene combined with irradiation group were set to compare the differences of three different gene expressions. The most active effects were found in the group of Hypoxia irradiation combined with β-elemene. In this group, the mTOR gene, HIF-1α gene, Survivin gene expressions were all down-regulated when compared with the single treatment groups, and there were significantly statistical differences.
Keywords:
A549; HIF-1α; Rhizoma Curcumae; Survivin; mTOR; β-elemene.
MeSH terms
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Adenocarcinoma / drug therapy
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Adenocarcinoma / metabolism*
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Adenocarcinoma / radiotherapy
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Adenocarcinoma of Lung
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Antineoplastic Agents, Phytogenic / pharmacology
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Antineoplastic Agents, Phytogenic / therapeutic use
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Cell Line, Tumor
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Chemotherapy, Adjuvant
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Curcuma / chemistry*
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Down-Regulation
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Gene Expression / drug effects
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Humans
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Hypoxia
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Hypoxia-Inducible Factor 1, alpha Subunit / genetics
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Hypoxia-Inducible Factor 1, alpha Subunit / metabolism*
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Inhibitor of Apoptosis Proteins / genetics
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Inhibitor of Apoptosis Proteins / metabolism*
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Lung Neoplasms / drug therapy
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Lung Neoplasms / metabolism*
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Lung Neoplasms / radiotherapy
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Phytotherapy
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Plant Extracts / pharmacology*
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Plant Extracts / therapeutic use
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Reverse Transcriptase Polymerase Chain Reaction
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Sesquiterpenes / pharmacology*
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Sesquiterpenes / therapeutic use
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Survivin
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TOR Serine-Threonine Kinases / genetics
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TOR Serine-Threonine Kinases / metabolism*
Substances
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Antineoplastic Agents, Phytogenic
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BIRC5 protein, human
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Hypoxia-Inducible Factor 1, alpha Subunit
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Inhibitor of Apoptosis Proteins
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Plant Extracts
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Sesquiterpenes
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Survivin
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beta-elemene
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MTOR protein, human
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TOR Serine-Threonine Kinases