Antifungal activity of Microgramma vacciniifolia rhizome lectin on genetically distinct Fusarium oxysporum f. sp. lycopersici races

Appl Biochem Biotechnol. 2014 Jan;172(2):1098-105. doi: 10.1007/s12010-013-0600-9. Epub 2013 Oct 20.

Abstract

Fusarium oxysporum f. sp. lycopersici races 1, 2, and 3 deteriorate tomato crops since they cause a vascular wilt. Lectins are carbohydrate-binding proteins with hemagglutinating and antifungal activities. This work reports that Microgramma vacciniifolia rhizome lectin (MvRL) inhibits F. oxysporum f. sp. lycopersici race 3 growth (61 %) more intensely than of races 1 (55 %) and 2 (45 %). The hemagglutinating activity of MvRL was inhibited by glycoprotein preparations from mycelia of races 1, 2, and 3, and these data indicate that lectin carbohydrate-binding sites recognized glycosylated molecules from races. Inter-simple sequence repeat (ISSR) marker system showed that race 3 is genetically distinct from races 1 and 2, and thus the highest sensitiveness of F. oxysporum f. sp. lycopersici race 3 to MvRL may be due to molecular characteristics of this race.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antifungal Agents / pharmacology*
  • Electrophoresis, Polyacrylamide Gel
  • Ferns / chemistry*
  • Fungal Proteins / metabolism
  • Fusarium / drug effects*
  • Fusarium / genetics*
  • Fusarium / growth & development
  • Genetic Variation
  • Microbial Sensitivity Tests
  • Plant Lectins / pharmacology*
  • Rhizome / chemistry*

Substances

  • Antifungal Agents
  • Fungal Proteins
  • Plant Lectins