Establishment of tracking system for West Nile virus entry and evidence of microtubule involvement in particle transport

Yoshinori Makino; Tadaki Suzuki; Rie Hasebe; Takashi Kimura; Akihiko Maeda; Hidehiro Takahashi; Hirofumi Sawa

doi:10.1016/j.jviromet.2013.10.002

Establishment of tracking system for West Nile virus entry and evidence of microtubule involvement in particle transport

J Virol Methods. 2014 Jan:195:250-7. doi: 10.1016/j.jviromet.2013.10.002. Epub 2013 Oct 16.

Authors

Yoshinori Makino¹, Tadaki Suzuki, Rie Hasebe, Takashi Kimura, Akihiko Maeda, Hidehiro Takahashi, Hirofumi Sawa

Affiliation

¹ Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan.

PMID: 24140187
DOI: 10.1016/j.jviromet.2013.10.002

Abstract

West Nile virus (WNV) is one of flaviviruses and has emerged recently in the United States as a significant cause of viral encephalitis. Although cellular entry of WNV is important for viral pathogenesis, its mechanisms have not been elucidated fully. To explore the entry mechanisms, a virus-particle tracking system in live cells by using fluorescently labeled subviral particles (SVPs) and time-lapse epifluorescence microscopy was established. This study revealed that, following cellular entry, SVP movements could be divided into two phases: early (slow movement) and late (fast movement) phase. Moreover, fast viral particle movements at the late phase correlated with SVP-microtubule association.

Keywords: Microtubules; Viral entry; West Nile virus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Humans
Microscopy, Fluorescence / methods*
Microtubules / metabolism*
Staining and Labeling / methods*
Virology / methods*
Virus Internalization*
West Nile virus / physiology*