Resource pulses in the world's hot deserts are driven largely by rainfall and are highly variable in both time and space. However, run-on areas and drainage lines in arid regions receive more water more often than adjacent habitats, and frequently sustain relatively high levels of primary productivity. These landscape features therefore may support higher biotic diversity than other habitats, and potentially act as refuges for desert vertebrates and other biota during droughts. We used the ephemeral Field River in the Simpson Desert, central Australia, as a case study to quantify how resources and habitat characteristics vary spatially and temporally along the riparian corridor. Levels of moisture and nutrients were greater in the clay-dominated soils of the riverine corridor than in the surrounding sand dunes, as were cover values of trees, annual grasses, other annual plants and litter; these resources and habitat features were also greater near the main catchment area than in the distal reaches where the river channel runs out into extensive dune fields. These observations confirm that the riverine corridor is more productive than the surrounding desert, and support the idea that it may act as a refuge or as a channel for the ingress of peri-desert species. However, the work also demonstrates that species diversity of invertebrates and plants is not higher within the river corridor; rather, it is driven by rainfall and the accompanying increase in annual plants following a rain event. Further research is required to identify the biota that depend upon these resource pulses.