A molecular method for assessing the effects of potential contaminants on the rate of zebrafish (Danio rerio) development

Environ Toxicol Chem. 2014 Jan;33(1):238-42. doi: 10.1002/etc.2428. Epub 2013 Nov 20.

Abstract

Monitoring expression of the developmentally regulated genes shh, sox2, and tnnt1 by reverse transcriptase polymerase chain reaction (RT-PCR) allows determination of the rate of embryogenesis in zebrafish (Danio rerio) embryos without direct visual observation. The utility of combining this approach and morphological methods during toxicity studies was demonstrated with embryos developing at either 28.5 °C or 24.5 °C and with embryos exposed to sublethal doses of silver nanoparticles.

Keywords: Aquatic Toxicology; Biomarkers; Developmental Toxicity; Genetic Toxicology; Nanoparticles.

MeSH terms

  • Animals
  • Embryonic Development / drug effects
  • Gene Expression Regulation, Developmental / drug effects*
  • Hedgehog Proteins / genetics
  • Hot Temperature
  • Metal Nanoparticles / toxicity*
  • SOX Transcription Factors / genetics
  • Silver / toxicity*
  • Stress, Physiological
  • Troponin T / genetics
  • Zebrafish / genetics*
  • Zebrafish / growth & development
  • Zebrafish Proteins / genetics

Substances

  • Hedgehog Proteins
  • SOX Transcription Factors
  • Shha protein, zebrafish
  • Sox2 protein, zebrafish
  • Troponin T
  • Zebrafish Proteins
  • Silver