Abstract
A total of 58 isolates of β-glucosidase-producing microorganisms were isolated from soil around the wild ginseng roots under forest using Esculin-R2A agar. Among these isolates, strain GS33 showed a strong ability to convert ginsenosides Rb1, Rb2, Rc, and Rd into F2, Rg3, C-K, and convert ginsenoside Rg1 into Rh1, and F1. Fermented ginseng products can inhibit ES-2 cells growth and the IC₅₀ value was 0.73 mg ml⁻¹. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain GS33 belongs to the genus Penicillium and is most closely related to Penicillium simplicissimum (99 %).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents / metabolism*
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Cell Line, Tumor
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Cluster Analysis
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DNA, Fungal / chemistry
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DNA, Fungal / genetics
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DNA, Ribosomal Spacer / chemistry
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DNA, Ribosomal Spacer / genetics
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Female
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Fermentation
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Ginsenosides / metabolism
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Humans
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Inhibitory Concentration 50
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Panax / chemistry*
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Panax / microbiology
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Penicillium / classification
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Penicillium / isolation & purification
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Penicillium / metabolism*
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Phylogeny
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Plant Extracts / metabolism*
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Plant Roots / microbiology
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Sequence Analysis, DNA
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Soil Microbiology
Substances
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Antineoplastic Agents
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DNA, Fungal
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DNA, Ribosomal Spacer
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Ginsenosides
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Plant Extracts