The characteristics of emotional expression in everyday life vary significantly depending on one's life experiences, role in society, and other factors, especially special occupation experiences. We focus on the nursing experience. The purpose of this study is to establish a method to discriminate voice of nurse's emotion. We performed the pronunciation experiment at first and through the sound analysis, extracted the effective speech parameters. For make clear of the differences of these parameters between emotions and discriminate emotions, we performed the multiple comparisons and discriminant analysis. In the result, found that the speech parameters different between emotions and these affected parameters are different at two groups based on nursing experience. In four emotions of our study, the Sadness is easy discriminant emotion; the Joy is most difficult to discriminate. Through the discriminant analysis, found two stable speech parameters.