Sodium is one of the essential indicators of cell viability in vivo. Regarding extant literature, sodium MRI is widely employed for various studies, including research on tumors, strokes, and neurocognition, because it can non-invasively provide data of physiological metabolism in vivo. Currently, strokes are among the 3 leading causes of death worldwide, and can be categorized as hemorrhagic or ischemic. Approximately 70% to 80% of stroke patients experience an ischemic stroke. Although numerous relevant studies have focused on larger animal models, such as rats, cats, and nonhuman primates, literature that employs mice experimental models is scarce. In this study, we conducted sodium MRI on the brains of mice after an ischemic stroke to observe sodium signal variations in the brain following this type of stroke. The findings indicated that the sodium signals in the brain regions affected by stroke were 2.3 times stronger than those in the lateral ventricles.