Ultrasound stimulus to enhance the bone regeneration capability of gelatin cryogels

Annu Int Conf IEEE Eng Med Biol Soc. 2013:2013:846-9. doi: 10.1109/EMBC.2013.6609633.

Abstract

In the present study, gelatin-based cryogels have been seeded with human SAOS-2 osteoblasts. In order to overcome the drawbacks associated with in vitro culture systems, such as limited diffusion and inhomogeneous cell-matrix distribution, this work describes the application of ultrasounds (average power, 149 mW; frequency, 1.5 MHz) to physically enhance the cell culture in vitro. The results indicate that the physical stimulation of cell-seeded gelatin-based cryogels upregulates the bone matrix production.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Regeneration / drug effects
  • Bone Regeneration / physiology*
  • Cattle
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Cryogels / pharmacology*
  • Extracellular Matrix / drug effects
  • Extracellular Matrix / metabolism
  • Gelatin / pharmacology*
  • Humans
  • Hydrogels / pharmacology
  • Microscopy, Electron, Scanning
  • Osteoblasts / cytology
  • Osteoblasts / drug effects
  • Porosity / drug effects
  • Tissue Scaffolds / chemistry
  • Ultrasonics*
  • X-Ray Microtomography

Substances

  • Cryogels
  • Hydrogels
  • Gelatin