Abstract
In the present study, gelatin-based cryogels have been seeded with human SAOS-2 osteoblasts. In order to overcome the drawbacks associated with in vitro culture systems, such as limited diffusion and inhomogeneous cell-matrix distribution, this work describes the application of ultrasounds (average power, 149 mW; frequency, 1.5 MHz) to physically enhance the cell culture in vitro. The results indicate that the physical stimulation of cell-seeded gelatin-based cryogels upregulates the bone matrix production.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Bone Regeneration / drug effects
-
Bone Regeneration / physiology*
-
Cattle
-
Cell Line, Tumor
-
Cell Proliferation / drug effects
-
Cryogels / pharmacology*
-
Extracellular Matrix / drug effects
-
Extracellular Matrix / metabolism
-
Gelatin / pharmacology*
-
Humans
-
Hydrogels / pharmacology
-
Microscopy, Electron, Scanning
-
Osteoblasts / cytology
-
Osteoblasts / drug effects
-
Porosity / drug effects
-
Tissue Scaffolds / chemistry
-
Ultrasonics*
-
X-Ray Microtomography
Substances
-
Cryogels
-
Hydrogels
-
Gelatin